DTT is used as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen.

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation …

Dithiothreitol (DTT) is extensively applied in chemical peptide synthesis and biochemical preparations of thiol proteins. It is also used in protein chemistry studies, such as protein folding and enzyme activity. …

Dithiothreitol | C4H10O2S2 | CID 439196 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier …

3 days ago · Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent . Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric ('sister') compound, …

DTT operates through thiol-disulfide exchange reactions. It possesses two thiol (-SH) groups that can donate electrons to disulfide bonds (S-S) in proteins, reducing them to free thiol groups.

dithiothreitol is a two-step reaction. First, DTT forms a mixed disulfide with the target molecule in a reversible reaction, reducing one . ulfur to the corresponding sulfhydryl. In the second step, …

This DTT reagent contains no detectable DNase or RNase activity and is suitable for use in molecular biology or protein biochemistry applications that require reduction of protein disulfi de bonds.

A staple of biochemistry and protein labs, dithiothreitol (DTT) was introduced in 1963. Less volatile and less odorous than the commonly used thiol β-Mercaptoethanol, DTT is a highly efficient and versatile …

DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).